Probing Origins of Molecular Interactions Stabilizing the Membrane Proteins Halorhodopsin and Bacteriorhodopsin
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چکیده
منابع مشابه
Probing the energy landscape of the membrane protein bacteriorhodopsin.
The folding and stability of transmembrane proteins is a fundamental and unsolved biological problem. Here, single bacteriorhodopsin molecules were mechanically unfolded from native purple membranes using atomic force microscopy and force spectroscopy. The energy landscape of individual transmembrane alpha helices and polypeptide loops was mapped by monitoring the pulling speed dependence of th...
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The split-Ubiquitin (split-Ub) technique was used to map the molecular environment of a membrane protein in vivo. Cub, the C-terminal half of Ub, was attached to Sec63p, and Nub, the N-terminal half of Ub, was attached to a selection of differently localized proteins of the yeast Saccharomyces cerevisiae. The efficiency of the Nub and Cub reassembly to the quasi-native Ub reflects the proximity...
متن کاملPhase Behavior and Interactions of the Membrane-Protein Bacteriorhodopsin
We present a synchrotron x-ray diffraction study of melting in stacks of two-dimensional crystalline arrays of the membrane protein bacteriorhodopsin. Two distinct regimes have been found as a function of the intermembrane distance d. In the “coupled” regime for d , 250 Å the temperature sTmd of the melting transition decreases with increasing d, demonstrating the effect of the repulsive membra...
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ژورنال
عنوان ژورنال: Structure
سال: 2005
ISSN: 0969-2126
DOI: 10.1016/j.str.2004.12.005